Comprehensive GC/MS Profiling of Volatile Organic Compounds in Whole and Glandular Saliva Using MonoTrap Micro-Extraction.

ABSTRACT:

Background/objectives

Salivary volatile organic compounds (VOCs) are promising noninvasive biomarkers for a wide range of diseases. While glandular saliva, secreted by salivary glands, is a relatively pure biofluid, whole saliva is a complex mixture containing oral microbiota, food debris, and desquamated epithelial cells. Therefore, a comprehensive comparison of the VOC profiles of these two types of saliva is essential to identify biologically relevant compounds. In this study, we aimed to establish a reliable method for VOC profiling from small saliva volumes and identify VOCs that reflect the biological differences between glandular and whole saliva.

Methods

We developed a protocol combining MonoTrap extraction with dichloromethane, allowing the analysis of VOCs from jus

SUBMITTER: Mori A 

PROVIDER: S-EPMC12654116 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature