Quantitative Estimation of Low-Abundance Targets in Engineered Systems and Environmental Samples: Comparative Study Between Droplet Digital PCR and Real-Time PCR.

ABSTRACT: Real-Time PCR (qPCR) is an extensively used biomolecular tool for the detection and quantification of nucleic acids for a variety of applications, spanning from clinical to environmental settings. However, qPCR relies on an external calibration curve and can be susceptible to inhibition caused by pollutants that are commonly found in environmental samples. More recently, droplet digital PCR (ddPCR) was proven to be the method of choice for detection and quantification when a target is present at a low abundance. While it has been extensively utilized in clinical studies, only a small amount of data is available for complex samples, which are often characterized by a low target concentration and high abundances of non-target and PCR inhibitors. In this study, ddPCR and qPCR assays were perf

SUBMITTER: Ayala Alban A 

PROVIDER: S-EPMC12654130 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature