Integrated Genomic and Phenotypic Analysis of <i>Bacillus safensis</i> LG01 Highlights Its Prospects in Biotechnology and Biocontrol.

ABSTRACT: Bacillus safensis strains have emerged as versatile microbial platforms for bioproduction, combining the benefits of probiotic utility and biocontrol. In this study, we describe the isolation and in-depth characterization of a previously unreported B. safensis strain, LG01. The genome of this strain comprises a circular chromosome encoding 13 secondary metabolite biosynthetic gene clusters, 144 carbohydrate-active enzymes, 2 antibiotic resistance loci, and 1 prophage region, indicative of strong antimicrobial and metabolic capacity. Its protein secretion systems support nutrient acquisition, colonization, quorum sensing, and antibiotic synthesis. Our phenotypic assays confirmed the antifungal and antibacterial activity, proteolytic and cellulolytic functions, and robust biofi

SUBMITTER: Yang L 

PROVIDER: S-EPMC12654287 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature