Identification of Three New Rugose Small Colony Variants from a <i>Pseudomonas aeruginosa</i> Biofilm.

ABSTRACT: Pseudomonas aeruginosa is a Gram-negative, pathogenic, bacterium that produces biofilms comprising phenotypically distinct cell subpopulations. When separating and characterizing a single P. aeruginosa PA14 biofilm, three novel rugose small colony variants (RSCVs) (denoted RSCV_1, RSCV_2, and RSCV_3) were discovered. Characteristics of these stationary phase RSCVs differed between stationary phase wild-type (WT) PA14, between the PA14 biofilm subpopulations, and between the RSCVs themselves. The observed phenotypic changes in the RSCVs included differences in cellular morphology, exopolysaccharide production, biosynthesis of virulence factors, biofilm formation, and antibiotic tolerance. Stationary phase cell surface-associated molecules on the RSCVs were differently ionized

SUBMITTER: Smartnick BK 

PROVIDER: S-EPMC12654532 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature