Negative Paper Spray Ionization Mass Spectrometry for the Determination of Endocrine-Disrupting Chemicals with Application to Paraben Analysis in Cosmetics.

ABSTRACT: Paper spray ionization mass spectrometry (PSI-MS) enables rapid analysis with minimal sample preparation, yet negative-ion mode performance has been limited by poor sensitivity and unstable signals, similar to conventional electrospray ionization. In this study, we optimized negative PSI tandem MS (MS/MS) for twelve endocrine-disrupting chemicals (EDCs) and related biomarkers-including bisphenols, phthalates, parabens, and substituted phenols-used as model analytes. A systematic solvent and additive screen identified 1 mM ammonium fluoride in methanol and 0.1% ammonium hydroxide in 9:1 MeOH/carbon tetrachloride as optimal conditions, providing enhanced deprotonated-ion intensities and improved stability. Calibration curves generated under these conditions showed excellent linearity, with l

SUBMITTER: Cho S 

PROVIDER: S-EPMC12654611 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature