Outer Membrane Vesicles, Lipidome, and Biofilm Formation in the Endophyte <i>Enterobacter Cloacae</i> SEA01 from <i>Agave Tequilana</i>.

ABSTRACT: Bacterial outer-membrane vesicles (OMVs) mediate stress tolerance, biofilm formation, and interkingdom communication, but their role in beneficial endophytes remains underexplored. We isolated 11 non-redundant isolates associated with Bacillus, Enterococcus, Kosakonia and Kocuria from Agave tequilana seeds, identified by MALDI-TOF MS and 16S rRNA gene sequencing. We focused on the catalase-negative Enterobacter cloacae SEA01, which exhibits plant-promoting traits and support agave growth under nutrient-poor microcosms. In addition, this endophyte produces OMVs. Time-resolved SEM documented OMV release and cell aggregation within 9 h, followed by mature biofilms at 24 h with continued vesiculation. Purified OMVs (≈80-300 nm) contained extracellular

SUBMITTER: Prieto KR 

PROVIDER: S-EPMC12654736 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature