Genomic Surveillance and Resistance Profiling of Multidrug-Resistant <i>Acinetobacter baumannii</i> Clinical Isolates: Clonal Diversity and Virulence Insights.

ABSTRACT: Acinetobacter baumannii is a multidrug-resistant opportunistic pathogen that poses critical challenges in hospital settings due to its environmental resilience and high resistance to antibiotics. Genomic surveillance has become essential for identifying transmission patterns, guiding antimicrobial stewardship, and informing infection control policies. We conducted whole-genome sequencing on 44 A. baumannii isolates collected between 2022 and 2023 from diverse wards in an Italian hospital. Illumina-based sequencing was followed by a comprehensive bioinformatics pipeline, including genome assembly, taxonomic validation, MLST, SNP-based phylogeny, pan-genome analysis, antimicrobial resistance (AMR) gene profiling, and virulence factor prediction. Most isolates were classified as

SUBMITTER: Ristori MV 

PROVIDER: S-EPMC12654848 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature