Theoretical-Experimental Analysis to Elucidate the Mechanism of Action of Novel Anabolic Agents.

ABSTRACT: Research into muscle tissue pathologies offers great opportunities for new pharmaceutical agents. Current therapies, including corticosteroids and immunosuppressants, have limited efficacy and significant adverse effects. In this context, steroidal hydrazone compound 4d was investigated for its ability to promote muscle growth and regeneration as a potential anabolic and regenerative modulator. Flow cytometry analysis showed that 4d significantly increases cell populations in S phase, indicating a strong proliferative stimulus in pathways regulated by TNF-α, AKT, MAFbX, and SMAD2/3. Molecular docking studies showed that 4d shares strong interactions with the known MasR agonist (EP-2825), exhibiting a higher predicted binding affinity. Furthermore, 4d demonstrate

SUBMITTER: Quiroga I 

PROVIDER: S-EPMC12654979 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature