Investigation of Antithrombotic Activity and In Vivo Effective Forms of Kaempferitrin Using FeCl₃-Induced Rat Arterial Thrombosis and UHPLC-Q-Exactive Orbitrap MS.

ABSTRACT: Kaempferitrin (KAE) is a natural flavonol dirhamnopyranoside with various pharmacological activities, isolated from the antithrombotic fraction of Celastrus orbiculatus Thunb. This study aimed to investigate the antithrombotic activity and "effective forms" of KAE. The results showed that KAE significantly prolonged rabbit plasma recalcification time in vitro. In the FeCl₃-induced rat arterial thrombosis model, KAE demonstrated antithrombotic effects by inhibiting coagulation, platelet aggregation, and fibrinolysis, with a lesser risk of bleeding compared to aspirin. KAE was orally administered to rats, and a total of 192 metabolites were characterized. These included 25 phase I metabolites, 8 hydroxylated and methylated metabolites, 57 sulfated metabolites, 74 glucuronid

SUBMITTER: Zhou J 

PROVIDER: S-EPMC12655163 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature