Enhancing Phenanthrene Degradation by <i>Burkholderia</i> sp. FM-2 with Rhamnolipid: Mechanistic Insights from Cell Surface Properties and Transcriptomic Analysis.

ABSTRACT: Various surfactants have been applied for the remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated environments, but their roles in bioremediation remain controversial. This study focused on rhamnolipid (a typical surfactant) and Burkholderia sp. FM-2 (a high-efficiency phenanthrene-degrading bacterium), investigating its effects on phenanthrene solubilization and biodegradation by analyzing cell surface characteristics and gene expression differences. Results showed that low concentrations of rhamnolipid (20-120 mg/L) promoted phenanthrene degradation, while high concentration (400 mg/L) exerted an inhibitory effect. At 20-56 mg/L, rhamnolipid altered the bacterial surface morphology and functional groups, facilitated lipopolysaccharide release, enhanced cell surface h

SUBMITTER: Zhai Y 

PROVIDER: S-EPMC12655266 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature