Raw Milk as a Source of <i>Campylobacter</i> Infection: Isolation and Molecular Identification of <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> in Ecuador.

ABSTRACT: The consumption of raw milk has been demonstrated to carry a potential risk of transmission of Campylobacter spp., with Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) being the major causes for foodborne gastroenteritis cases. The present study assessed the prevalence and species distribution of Campylobacter spp. in 633 raw milk samples collected over a one-year climatic cycle from small, medium, and large producers in Pichincha and Manabí, Ecuador. Samples were augmented and analyzed by qPCR for Campylobacter spp., while species identification was performed by duplex PCR and confirmed by 16S rDNA sequencing. The average prevalence of Campylobacter spp. was 49.9% (316/633), with a higher detection rate in Manabí (57.6%, 182/316)

SUBMITTER: Padilla-Cerda A 

PROVIDER: S-EPMC12655607 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature