Optimization by the 4S Sequential Experimental Design Process of a Competitive Lateral Flow Immunoassay Device for the Detection of Aflatoxin B1.

ABSTRACT: Aflatoxin B1 (AFB1) is a highly toxic and carcinogenic compound produced by certain fungi (e.g., Aspergillus flavus and Aspergillus parasiticus). Rapid and ultra-sensitive detection methods for AFB1 in various commodities are in high demand. This study aimed to enhance the sensitivity of a competitive lateral flow immunoassay (LFIA) for AFB1 detection by leveraging a previously developed experimental design strategy, named 4S. This approach comprises four phases-START, SHIFT, SHARPEN, and STOP-and involves the analysis of two reference conditions: NEG (0 ng/mL AFB1) and POS (1 ng/mL AFB1). By generating and overlaying response surfaces, regions of optimal NEG signal and POS/NEG signal ratio (IC%) were identified. Four variables were optimized: two related to the labeled antib

SUBMITTER: Cavalera S 

PROVIDER: S-EPMC12656006 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature