CRISPR-Cas9-Mediated Knockout of <i>MLO3</i> Confers Enhanced Resistance to Reniform Nematode in Upland Cotton.

ABSTRACT: Upland cotton (Gossypium hirsutum L.) is a major global commodity crop whose production is threatened by the reniform nematode (Rotylenchulus reniformis Linford and Oliveira), a plant-parasitic pest that causes substantial yield losses. Host-plant resistance offers a sustainable management strategy, but currently available resistant cotton cultivars provide only partial protection and often require supplemental control methods. In this study, Clustered Regularly Interspaced Palindromic Repeats (CRISPR)-CRISPR-associated 9 (Cas9) gene editing was used to generate targeted knockouts of Mildew Resistance Locus O (GhiMLO3) in cotton and assess its role in resistance to R. reniformis. Four independent knockout lines (A1, D3, E1, and P3) were developed, confirm

SUBMITTER: Kangben F 

PROVIDER: S-EPMC12656067 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature