Unraveling the Mechanisms of <i>Biebersteinia heterostemon</i> in Improving Hyperlipidemia: A Network Pharmacology, Molecular Docking, and In Vitro Validation in HepG2 Cells.

ABSTRACT: Biebersteinia heterostemon is a traditional Tibetan medicine known for its antioxidant, hypoglycemic, and anti-atherosclerotic properties. However, its therapeutic effects and mechanisms in the treatment of hyperlipidemia remain unclear. In this study, the ethyl acetate extract of B. heterostemon (BHEE) was first identified as the most effective lipid-lowering fraction through its inhibitory activity on pancreatic lipase and cholesterol esterase. Chemical characterization of BHEE by UHPLC-MS/MS revealed 108 compounds. Network pharmacology and molecular docking analyses were then employed to predict key active components and signaling pathways involved in BHEE's lipid-lowering effects. A total of 50 active components and 623 targets were selected from the PubChem, SwissADME, a

SUBMITTER: Shen X 

PROVIDER: S-EPMC12656387 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature