Expression and Immunological Characterization of African Swine Fever Virus <i>EP153R</i> Protein for Serodiagnosis and Its Delivery via a Recombinant PRRSV Live Vector.

ABSTRACT: Background/Objectives: African Swine Fever (ASF), caused by the African Swine Fever Virus (ASFV), is a highly contagious and lethal disease in pigs, for which no recognized safe and effective vaccine is currently available. The ASFV EP153R gene, expressed during both early and late infection stages, exhibits strong protective potential. Utilizing advances in genetic engineering, recombinant PRRSV vector vaccines carrying ASFV exogenous genes were constructed. This study aims to prepare pEP153R-based polyclonal antibodies and an iELISA detection method using the constructed rPRRSV-EP153R as a specific target to verify the iELISA's specificity and effectiveness. Methods: A prokaryotic plasmid, pCold-TF-EP153R, was constructed to express protein in BL21 (DE3). The purifie

SUBMITTER: Luo M 

PROVIDER: S-EPMC12656761 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature