Characterization of Five Lytic Bacteriophages as New Members of the Genus <i>Mosigvirus</i>, Infecting Multidrug-Resistant Shiga Toxin-Producing <i>Escherichia coli</i> and Their Antibiofilm Activity.

ABSTRACT: The emergence of multidrug-resistant Shiga toxin-producing Escherichia coli (STEC) poses a major challenge to public health and necessitates the development of alternative antimicrobial strategies. This study aimed to isolate and characterize five lytic bacteriophages belonging to the genus Mosigvirus and evaluate their potential as biocontrol against MDR STEC strains and their biofilms. The five bacteriophages, designated vB_EcoM-pJBB (ΦB), vB_EcoM-pJBC (ΦC), vB_EcoM-pJBJ (ΦJ), vB_EcoM-pJBK (ΦK), and vB_EcoM-pJBL (ΦL), were isolated from sewage treatment plant samples using STEC ATCC 43895 as host. Biological characterization included host range determination against 19 MDR STEC strains, one-step growth analysis, environmental stability assays, bacteriolytic activity assessm

SUBMITTER: Na JB 

PROVIDER: S-EPMC12656860 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature