Characterization of Chemically-Induced Endogenous Retroviral Particles in the CHO-K1 Cell Line.

ABSTRACT: The Chinese hamster ovary K1 cell line (CHO-K1) constitutively produces retroviral-like particles (RVLPs) containing reverse transcriptase (RT) activity, which, thus far, have not been shown to be infectious. Since infectious retroviruses have been reported in other rodent species, this study was undertaken to investigate the presence of latent, infectious, endogenous retroviruses (ERVs) in CHO-K1 cells by using chemical induction assays and detection of activated virus using the highly sensitive, product-enhanced RT (PERT) assay, with subsequent infectivity analysis in cell lines of different species, including human. The results demonstrated activation of A-type and C-type retroviral particles based on transmission electron microscopy and increased production of cell-free RT-particles af

SUBMITTER: Mattson NB 

PROVIDER: S-EPMC12656867 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature