Characterization of a Phage-Encoded Depolymerase Against <i>Klebsiella pneumoniae</i> K30 Capsular Type and Its Therapeutic Application in a Murine Model of Aspiration Pneumonia.

ABSTRACT: Capsular polysaccharides are critical virulence factors of Klebsiella pneumoniae, enabling the bacterium to evade host immune recognition and exacerbate infection. Phage-derived depolymerases, which specifically degrade these capsular polysaccharides, are increasingly recognized as a highly promising strategy for the treatment of bacterial infections. In the present study, we isolated and characterized a lytic Klebsiella pneumoniae phage, named phiTH1, and sequenced its genome. The K30-type capsular polysaccharide was identified as the receptor for phiTH1 infection. A tail fiber protein with a pectate lyase domain, Dop5, was then recognized as a potential K30-type depolymerase. Therefore, the recombinant protein Dop5 was expressed in Escherichia coli and purified, and

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12656996 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature