Single-cell transcriptomic dissection of two waves of endothelial-hematopoietic transition in a murine yolk sac.

ABSTRACT: The yolk sac drives vertebrate embryonic hematopoiesis through primitive hematopoiesis and endothelial-to-hematopoietic transition (EHT) waves. However, dynamic cellular and molecular changes during EHT of the yolk sac remain to be elucidated. We built a comprehensive atlas of early endothelial and hematopoietic development in the yolk sac by integrating single-cell transcriptomic data from mouse embryos (E6.75-E11.0). Focusing on the yolk sac (E7.5-E9.5), we established a refined atlas capturing key cell populations of EHT in the yolk sac. This enabled the identification of distinct hemogenic endothelial cell (HEC) subpopulations and revealed 2 fundamentally distinct waves of yolk sac hemogenesis via EHT that differed in temporal emergence, cellular origin, molecular signature, and lineag

SUBMITTER: Chen Z 

PROVIDER: S-EPMC12657049 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature