Comparative study of pre- and post-mortem perfusion of fixative for the quality of neuronal tissue preparation.

ABSTRACT: Ante-mortem transcardiac perfusion of a fixative agent is generally recommended for quality preparations for cerebral histology, ensuring rapid and deep penetration in the tissue to preserve the most fragile brain structures. Despite being performed under anesthesia and with proper analgesia, this procedure is cumbersome for the experimenter and raises ethical questions. Recently, alternative protocols have been proposed based on prior animal euthanasia followed by an injection of a fixative agent into the circulation. These so-called post-mortem perfusion protocols should in theory ensure an equivalent quality of tissue fixation, without exposing live animals to a procedure. Before adopting this new method, it is necessary to validate that sample quality is equivalent, ensuring the validi

SUBMITTER: Meyer-Dilhet G 

PROVIDER: S-EPMC12657237 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature