Cx47 Phosphorylation Exacerbates White Matter Damage and Kainic Acid Induced Epilepsy.

ABSTRACT:

Aims

Growing evidence implicates dysfunctional myelin in the pathogenesis of temporal lobe epilepsy (TLE). Connexin 47 (Cx47), an oligodendrocytic gap junction protein, maintains myelin integrity. This study investigates the role of Cx47 in myelin impairment and seizure progression in TLE.

Methods

Cx47 and phosphorylated Cx47 (p-Cx47) expression was analyzed in human and mouse TLE brain tissues via Western blot and immunofluorescence. Candidate Cx47 phosphorylation kinases revealed by single-cell RNA sequencing were validated through immunofluorescence, protein docking, and co-immunoprecipitation. TLE mice were treated with the CaMKII inhibitor KN93 to evaluate its effects on demyelination and seizure burden.

Results

In a experimental mouse model, phosphorylated Cx47

SUBMITTER: Li Y 

PROVIDER: S-EPMC12657264 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature