Negative feedback regulation of CHK2 in VIN18 raft cultures naturally infected with HPV18.

ABSTRACT: The life cycle of human papillomavirus (HPV) is intricate, and a lack of appropriate in vitro models for natural HPV infection has led to a dearth of effective treatments for infection and related tumors. The HPV life cycle is strictly dependent on the differentiation of epithelial cells. Therefore, in our previous study, we used conditional reprogramming(CR) technology to establish human vaginal intraepithelial neoplasia cell infected with HPV18 naturally (VIN18) and verified that HPV18 completes its viral life cycle in these cells. This article refers to it as VIN18. In this study, we utilize VIN18 to establish a 3D differentiation model that facilitates a time gradient of infection within raft-like organotypic cultures.Our findings clarify previous understanding of the interaction betwe

SUBMITTER: Wu M 

PROVIDER: S-EPMC12657343 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature