Establishment of a rapid split-root assay in hydroponic conditions for eight upland cotton varieties.

ABSTRACT: Upland cotton (Gossypium hirsutum L.) is a major crop in the United States. Understanding how cotton roots develop and respond to abiotic and biotic factors is crucial for improving nutrient acquisition, enhancing crop resilience under stress, and optimizing overall crop production. Split-root techniques have been developed for numerous plant species, providing a controlled framework for monitoring root development, and investigating systemic and local plant responses to various environmental factors. However, a standardized cotton-specific protocol optimized for laboratory studies has yet to be established. This protocol facilitates the rapid establishment of split-root systems in eight upland cotton varieties within four weeks after germination. This is accomplished by cutting the

SUBMITTER: Jamison BJ 

PROVIDER: S-EPMC12657376 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature