Tissue-resident macrophages co-develop with myocardial tissue in human induced pluripotent stem cell-derived organoids.

ABSTRACT: The heart is the first functional organ to develop during embryogenesis, forming in parallel with the vasculature and hematopoietic cell lineages. To advance our understanding of human cardiac development and disease, human induced pluripotent stem cell (iPSC)-derived cardiomyocytes offer a promising in vitro model. However, conventional 2D cultures lack the complexity required to recapitulate the intricate interactions of different cell types leading to fully functional and mature cardiac tissue. Here, we present a human iPSC-derived 3D organoid model that develops a functional myocardium composed of cardiomyocytes and fibroblasts, capable of spontaneous rhythmic contractions. The organoid is interspersed with a branched network of endothelium-lined cavities, endothelial cords and

SUBMITTER: Rockel AF 

PROVIDER: S-EPMC12657418 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature