NK cell-associated long non-coding RNAs reveal heterogeneity of colorectal cancer immune microenvironment.

ABSTRACT:

Introduction

Individuals diagnosed with colorectal cancer (CRC) frequently confront a grave prognosis and exhibit poor responses to conventional treatment regimens. Immunotherapy, notably modalities centered on natural killer (NK) cells, represents a burgeoning frontier in the management of CRC. This study developed a validated prognostic model using NK-associated long non-coding RNAs (lncRNAs) to predict CRC outcomes.

Methods

Integrating single-cell RNA-seq (GSE146771_Smartseq2) and TCGA-COAD/READ bulk transcriptomic data, we identified NK-specific genes and correlated lncRNAs. A multi-step analytical approach-including univariate Cox regression for preliminary screening, LASSO regression to minimize overfitting, and multivariate Cox regression for final model optimization-

SUBMITTER: Li Y 

PROVIDER: S-EPMC12657463 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature