Cold temperature enhances innate eosinophilic airway inflammation via transient receptor potential ankyrin1.

ABSTRACT:

Background and objective

Asthma exacerbations due to cold air exposure are well recognized; however, the underlying mechanisms remain unclear. We investigate the role of the transient receptor potential ankyrin1 (TRPA1) channel in cold air-induced aggravation of innate airway inflammation using a murine model of papain stimulation combined with cold air exposure.

Methods

Wild-type (WT) and Trpa1 knockout (KO) mice were treated intranasally with papain under different temperature conditions. Bronchoalveolar lavage fluid (BALF) and lung tissues were analyzed. The effects of the TRPA1 antagonist HC030031 were also evaluated. Additionally, human bronchial epithelial (HBE) cells were stimulated with papain and the TRPA1 agonist allyl isothiocyanate (AITC).

Results

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SUBMITTER: Dotake Y 

PROVIDER: S-EPMC12657488 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature