Redox partner adrenodoxin induces substrate binding to steroidogenic cytochrome P450 11B2 and 11A1 by promoting a conformational change.

ABSTRACT: In humans, cytochrome P450 (CYP) 11A1 and 11B2 play crucial roles in the steroidogenic pathway. CYP11A1 is involved in the first step in the steroidogenic pathway, converting cholesterol to pregnenolone, whereas CYP11B2 completes the final step in aldosterone synthesis. Each is a drug target for a distinct disease, and detailed information about enzyme kinetics and function could inform drug development. Both enzymes require the common redox partner adrenodoxin. Adrenodoxin allosterically modulates CYP11B2 function separate from its role in electron transfer. Herein, stopped-flow investigations establish that adrenodoxin is also an allosteric modulator of CYP11A1 substrate binding. For both enzymes, a kinetic modeling approach was used to elucidate the substrate binding mechanisms in the a

SUBMITTER: Loomis CL 

PROVIDER: S-EPMC12657753 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature