Additive-free hyaluronic acid-based bioink for 3D bioprinting of bone marrow microenvironments.

ABSTRACT: Bioprinting of soft tissues is an emerging technology with significant potential in regenerative medicine. It requires bioinks that mimic biochemical and physical properties of natural tissues and enable precise positioning of multiple cell types to allow their physiological interplay. Here, we describe the development of a bioink for bone marrow as an example of soft tissue. Bone marrow is the site of blood regeneration, driven by hematopoietic stem cells (HSCs) and relying on the orchestrated interplay of hematopoietic and stromal cells in a soft microenvironment. The bioink is based on hyaluronic acid (HA), dual-functionalized in a one-pot synthesis with alkyl side chains enhancing physical crosslinking via hydrophobic interactions and methacrylamide groups allowing covalent photo-cross

SUBMITTER: Naolou T 

PROVIDER: S-EPMC12657760 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature