The condensin I subunit Barren/CAP-H is essential for the structural integrity of centromeric heterochromatin during mitosis.

ABSTRACT: During cell division, chromatin undergoes structural changes essential to ensure faithful segregation of the genome. Condensins, abundant components of mitotic chromosomes, are known to form two different complexes, condensins I and II. To further examine the role of condensin I in chromosome structure and in particular in centromere organization, we depleted from S2 cells the Drosophila CAP-H homologue Barren, a subunit exclusively associated with condensin I. In the absence of Barren/CAP-H the condensin core subunits DmSMC4/2 still associate with chromatin, while the other condensin I non-structural maintenance of chromosomes family proteins do not. Immunofluorescence and in vivo analysis of Barren/CAP-H-depleted cells showed that mitotic chromosomes are able to condense but fail to reso

SUBMITTER: Oliveira RA 

PROVIDER: S-EPMC1265781 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature