Pachymic acid alleviates lipopolysaccharide-induced cystitis glandularis by inhibiting ferroptosis.

ABSTRACT: BackgroundCystitis glandularis is a metaplastic lesion of the bladder mucosa. The underlying pathogenesis of cystitis glandularis and the potential association between the therapeutic effects of pachymic acid and ferroptosis remain unclear.MethodsHuman bladder epithelial cells (SV40-immortalized human urothelial cells-1 (SV-HUC-1)) and Sprague-Dawley rat bladders were treated with lipopolysaccharide, with pachymic acid and ferrostatin-1 used as interventions. Cell viability was determined using the Cell Counting Kit-8 (CCK-8) assay. Levels of reactive oxygen species, glutathione, and malondialdehyde were quantified in both cells and tissues. Levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6 in rat bladder tissues were quantified using enzyme-linked immunosorbent assay ki

SUBMITTER: Tang Y 

PROVIDER: S-EPMC12657829 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature