Fine tuning wheat heading time through genome editing of transcription factor binding sites in Ppd-1 gene promoter.

ABSTRACT: Increasing the productivity and adaptability of agricultural plants often depends on optimizing heading time. To develop common wheat lines with accelerated heading and investigate its regulation, we targeted the PPD-1 genes, which control photoperiod sensitivity. Large deletions in the promoter regions of these genes are known to disrupt their expression, resulting in early heading. Using CRISPR/Cas9 genome editing, we generated wheat plants with mutations in the promoter regions of the Ppd-D1 and Ppd-B1 genes. These mutations included nucleotide substitutions, deletions, and insertions ranging from several to hundreds of base pairs, occurring within probable transcription factor binding sites, that may influence gene expression. Under short-day conditions, we assessed PPD-1 gene expressi

SUBMITTER: Kiseleva AA 

PROVIDER: S-EPMC12657949 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature