Identification of miR-940 and associated gene features as novel biomarkers for early detection of cerebral hemorrhage.

ABSTRACT: ICH, a severe stroke, causes neuronal death, neuroinflammation, and cerebral edema due to mitochondrial and immune dysfunction. The molecular mechanisms of secondary brain injuries are unclear, limiting effective therapies. This study used bioinformatics and experiments to explore miR-940's role in ICH. MitoDEGs were identified via MitoCarta 3.0, and key miRNAs predicted using TargetScan and miRDB. A collagenase-induced ICH rat model with antagomir knockdown was used for validation. Neuronal damage, mitochondrial proteins, and immune cell dynamics were assessed using histopathology, qRT-PCR, Western blotting, and flow cytometry. Bioinformatics identified seven MitoDEGs and miR-940. Functional analysis linked them to mitochondrial metabolism and neuroinflammation. Inhibiting miR-940 in vivo

SUBMITTER: Zhao X 

PROVIDER: S-EPMC12658068 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature