Development and evaluation of a hybrid capture-based NGS panel for comprehensive detection of respiratory pathogens.

ABSTRACT: Infectious diseases caused by diverse pathogens-including viruses, bacteria, and fungi-pose a significant diagnostic challenge, especially when conventional assays fail to identify the causative agents. Next-generation sequencing (NGS), particularly hybrid-capture-based NGS, offers a culture-independent approach for comprehensive pathogen detection directly from clinical samples. In this study, we developed a hybrid-capture-based NGS panel targeting 85 respiratory pathogens (62 viruses, 23 bacteria/fungi) and evaluated its analytical and clinical performance. Analytical validation using 50 pathogens demonstrated high sensitivity, with 10× genome coverage ≥ 96% in 32 of 36 viral targets and ≥ 95% in all 14 bacterial/fungal targets. The panel maintained ≥ 80% genome coverage at Ct values up

SUBMITTER: Kim JA 

PROVIDER: S-EPMC12658075 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature