Decoding the anti-inflammatory properties of sesquiterpene coumarins from the active fractions of Ferula assa-foetida through integrated experimental and in silico analyses.

ABSTRACT: Ferula assa-foetida known as Asafoetida has a long-established history in folk medicine as a therapeutically useful drug in many disorders. The current paper explored the in vivo and in vitro anti-inflammatory activities of the plant. Liquid ethanol crude extract was subjected to liquid-to-liquid extraction, and the fractions so obtained were evaluated against the carrageenan-induced acute paw edema model in rats, to determine the anti-inflammatory effects. The fractions that gave the greatest activity-ethyl acetate and hexane- afforded four sesquiterpene coumarins: auraptene (1), umbelliprenin (2), galbanic acid (3), and kamolonol (4). The overall structure was determined through detailed spectroscopic investigation, ¹H NMR, ¹³C NMR, DPET135, COSY, HSQC, HMBC, HRESIM

SUBMITTER: Alamri MA 

PROVIDER: S-EPMC12658108 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature