ICAT as an open-source platform for axial rotation and high-resolution imaging of organoids and living organisms.

ABSTRACT: In dynamic light microscopy applications, imaging specimens from multiple angles while maintaining controlled temperature conditions is crucial for comprehensive and accurate analysis. To address these challenges, we present iCAT, an open-source multifunctional accessory designed to enhance light microscopy. It enables specimen rotation along the axial plane, incorporates built-in modules for precise temperature control, features an integrated LED, and includes a camera for real-time specimen monitoring. It can be easily 3D-printed and assembled using readily available electrical components. Combined with any up-right microscope, this versatile device allows researchers to capture detailed images and videos of organoid cultures and live or fixed specimens, such as C. elegans, zebrafish, dr

SUBMITTER: Svoboda O 

PROVIDER: S-EPMC12658174 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature