Methylene blue reduces postoperative delirium in mice through neuroinflammation suppression and blood-brain barrier repair.

ABSTRACT: Postoperative delirium (POD) is a serious complication following anesthesia and surgery, with currently unclear pathogenesis and no effective treatments. In recent years, emerging evidence implicates that neuroinflammatory and blood-brain barrier (BBB) dysfunction play an important role in the pathogenesis of POD. Methylene blue (MB), an FDA-approved drug, has been reported to have anti-inflammatory and neuroprotective properties. We applied a POD model with a simple laparotomy to assess the POD-like behavior in adult mice. Behavioral tests were performed at 24 h preoperatively, 6 h and 24 h postoperatively. We evaluated neuroinflammation and the reactive states of microglia in the hippocampus and prefrontal cortex. We also evaluated the permeability of the BBB by detecting tight junction

SUBMITTER: Deng Y 

PROVIDER: S-EPMC12658177 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature