Optimizing DCD donor liver function with resveratrol during machine perfusion.

ABSTRACT: Extensive research has explored the use of machine perfusion to repair liver grafts, among these, interventions targeting mitochondrial repair are gaining attention due to its central role in ischemia-reperfusion injury and graft viability. Resveratrol, a mild natural polyphenol, is investigated here as a representative compound. Rat donor livers were subjected to 30 min of asystolic warm ischemia (DCD), followed by 2 h of either static cold storage (SCS), subnormothermic machine perfusion (SNMP) at 25 °C, or SNMP supplemented with resveratrol (SNMP-RES). All livers were subsequently reperfused under normothermic conditions for 1 h (NMP) with donor blood. Biochemical, transcriptomics RNA sequencing, inflammatory, and histological analyses were performed on perfusate samples and liver tissu

SUBMITTER: Jiao C 

PROVIDER: S-EPMC12658267 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature