NtCML19 Is Recruited by Tobacco to Interact With the Deacetylase Protein RsDN3377 of Rhizoctonia solani AG3-TB, Inhibiting Fungal Infection.

ABSTRACT: The necrotrophic fungal pathogen Rhizoctonia solani anastomosis group 3 (AG3-TB) is a major cause of global tobacco crop yield losses. Secreted proteins produced by filamentous fungi, as important virulence factors, play a core role in the interaction between plants and pathogens. In this study, we identified a secretory protein, RsDN3377, which localised to the intercellular space and induced cell death in Nicotiana benthamiana. Heterologous expression in Escherichia coli coupled with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry analysis confirmed RsDN3377 possessed deacetylase activity. In addition, RsDN3377 was an essential pathogenicity factor for mycelial development by double-stranded RNA-mediated gene silencing. Through yeast two-hybrid and bimolecula

SUBMITTER: Li X 

PROVIDER: S-EPMC12658376 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature