Single-Nucleus RNA-Seq Reveals Aerobic Exercise-Induced Remodeling of Spinal Cord Aging in Mice.

ABSTRACT: Spinal cord aging is a critical physiological process that compromises central nervous system (CNS) homeostasis and plasticity. Exercise, as a systemic intervention with broad health benefits, has been shown to delay neurodegeneration and preserve tissue function; however, its impact on dynamic cellular lineage evolution and intercellular communication within the aging spinal cord remains poorly characterized. In this study, we employed single-nucleus RNA sequencing (snRNA-seq) to construct a high-resolution cellular atlas of the mouse spinal cord under young, aged, and aerobic exercise-intervened conditions. By integrating unsupervised clustering, cell proportion analysis, pseudotime trajectory reconstruction, gene regulatory network (GRN) inference, and intercellular communication mappin

SUBMITTER: Du J 

PROVIDER: S-EPMC12658470 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature