Single-cell RNA sequencing data analysis of the inner ear in gentamicin-treated mice via intraperitoneal injection.

ABSTRACT:

Objective

This study aims to delineate the mechanisms through which intraperitoneal injection of gentamicin (GEN) influences the inner ear cells of mice by employing single-cell RNA sequencing (scRNA-seq) technology.

Methods

Eight-week-old Kunming mice were randomly assigned to three groups: a normal control group, a GEN group, and a GEN + dexamethasone (DEX) group. The mice received continuous intraperitoneal injections of the corresponding drugs for 10 days. Auditory brainstem response (ABR) was assessed to evaluate hearing threshold shifts, and cochlear tissues were harvested for scRNA-seq. The Seurat analysis workflow was employed for data quality control, dimensionality reduction clustering, and differential gene expression analysis.

Results

ABR results demonstra

SUBMITTER: Bao X 

PROVIDER: S-EPMC12658731 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature