Aggregation-Induced Excimer Emission-Controlled Ratiometric Fluorescence Assay for Determining Critical Micelle Concentration of Surfactants.

ABSTRACT: The critical micelle concentration (CMC) is a key physicochemical parameter that defines the onset of micelle formation in surfactant solutions. The determination of the CMC is essential for understanding surfactant behavior in various chemical, biological, and industrial applications. This study introduces a ratiometric fluorescence assay that exploits the distinct monomer-excimer emission behavior of pyrene-butyldipicolylamine (Py-DPA) to determine the CMC of various surfactants. Py-DPA forms self-assembled nanoaggregates in a buffered solution and exhibits strong aggregation-induced excimer emission. As the concentration of surfactant increases and micelle formation occurs, the dissolution of Py-DPA nanoaggregates induces a distinct fluorescence transition from exci

SUBMITTER: Kang S 

PROVIDER: S-EPMC12658812 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature