Synthesis, Characterization, and Biological Evaluation of Novel Azo-Based Imidazo[1,2‑<i>a</i>]pyridine Derivatives: In Vitro and In Silico Investigations.

ABSTRACT: In this study, a series of azo-linked imidazo-[1,2-a]-pyridine derivatives (4a-f) was synthesized and fully characterized using FTIR, NMR (¹H and ¹³C), and mass spectrometry. Structural and electronic properties were investigated through Density Functional Theory (DFT) at the B3LYP/6-311++G-(d,p) level, revealing key insights into charge distribution, molecular orbitals, and reactivity indices. The antibacterial and antibiofilm activities were assessed in vitro against Gram-positive and Gram-negative strains, including multidrug-resistant isolates. 4e exhibited the most potent antibacterial activity, with minimum inhibitory concentrations (MICs) of 0.5-1.0 mg/mL, and retained activity against resistant strains, showing MICs of 0.5-0.7 mg/mL

SUBMITTER: Azzouzi M 

PROVIDER: S-EPMC12658838 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature