D-amino acid oxidase suppresses hepatocellular carcinoma via oxidizing D-amino acids.

ABSTRACT:

Background

Patient-derived organoids provide a powerful platform for elucidating mechanisms of drug resistance and tumor evolution in hepatocellular carcinoma (HCC) and identifying novel therapeutic targets.

Methods

Transcriptomic sequencing was used to compare gene expression patterns between organoid-forming and non-forming HCC tissues, as well as between sorafenib-resistant organoids and sorafenib-sensitive counterparts. The TCGA-liver hepatocellular carcinoma (LIHC) cohort was used to screen for the key molecular drivers of HCC evolution from the overlapping differentially expressed genes. The effects of D-amino acid oxidase (DAO) on the growth, migration, invasion, apoptosis, and generation of H₂O₂ were evaluated in HepG2 and SK-Hep-1 cell lines an

SUBMITTER: Li Z 

PROVIDER: S-EPMC12659175 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature