Protective effects of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on radiation-induced epididymal cells damage via USP44/RBM14 axis.

ABSTRACT:

Background

Radiation-induced damage to reproductive tissues presents a major clinical concern with potentially lasting functional consequences.

Methods

Here, we investigate the protective function of extracellular vesicles derived from mouse bone marrow mesenchymal stem cells (mBMSCs-EVs) in mitigating ionizing radiation-induced injury in murine epididymal cells (mPC-1).

Results

We demonstrate that mBMSC-EVs significantly enhance cell viability and reduce apoptosis, oxidative stress, and DNA damage in irradiated mPC-1 cells. Besides, we identify the deubiquitinase USP44 as a key mediator enriched in mBMSC-EVs, which confers radioprotection by stabilizing the RNA-binding protein RBM14 through deubiquitination for mPC-1 cells. Silencing USP44 in mBMSCs or their EVs abro

SUBMITTER: Zhu L 

PROVIDER: S-EPMC12659287 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature