Unknown

Dataset Information

0

Cloning and characterization of the crystal protein-encoding gene of Bacillus thuringiensis subsp. yunnanensis.

ABSTRACT: Molecular cloning and characterization of a novel cry gene, cry32Aa, of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutella xylostella.

SUBMITTER: Balasubramanian P 

PROVIDER: S-EPMC126593 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Cloning and characterization of the crystal protein-encoding gene of Bacillus thuringiensis subsp. yunnanensis.

Balasubramanian Periasamy P   Jayakumar R R   Shambharkar Prashant P   Unnamalai N N   Pandian S Karutha SK   Kumaraswami N Selvamuthu NS   Ilangovan R R   Sekar Vaithilingam V  

Applied and environmental microbiology 20020101 1

Molecular cloning and characterization of a novel cry gene, cry32Aa, of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutell  ...[more]

Similar Datasets

Unknown Insecticidal toxins from Bacillus thuringiensis subsp. kenyae: gene cloning and characterization and comparison with B. thuringiensis subsp. kurstaki CryIA(c) toxins.
| S-EPMC182717 | biostudies-literature
Unknown Isolation and characterization of a novel insecticidal crystal protein gene from Bacillus thuringiensis subsp. aizawai.
| S-EPMC208042 | biostudies-other
Unknown Transcriptional regulation of the Bacillus thuringiensis subsp. thompsoni crystal protein gene operon.
| S-EPMC206974 | biostudies-other
Unknown Molecular characterization of two novel crystal protein genes from Bacillus thuringiensis subsp. thompsoni.
| S-EPMC205749 | biostudies-other
Unknown Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.
| S-EPMC167734 | biostudies-other
Unknown Insecticidal properties of a crystal protein gene product isolated from Bacillus thuringiensis subsp. kenyae.
| S-EPMC195296 | biostudies-other
Unknown Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus.
| S-EPMC167511 | biostudies-other
Unknown A novel Bacillus thuringiensis gene encoding a Spodoptera exigua-specific crystal protein.
| S-EPMC210793 | biostudies-other
Unknown Identification and characterization of three previously undescribed crystal proteins from Bacillus thuringiensis subsp. jegathesan.
| S-EPMC3648049 | biostudies-literature
Unknown Cloning and expression of two crystal protein genes, cry30Ba1 and cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus INA288.
| S-EPMC1538732 | biostudies-literature