Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy.

ABSTRACT:

Background

For patients with advanced hepatocellular carcinoma, sorafenib is considered a highly effective targeted molecular drug; however, sorafenib resistance limits its therapeutic efficacy. Hepatocellular carcinoma, a type of solid tumour, contains hypoxic and oxygen-rich regions within its internal structure. This study investigated the mechanism by which hypoxic hepatocellular carcinoma cells influence the sorafenib sensitivity of normoxic hepatocellular carcinoma cells through the secretion of hypoxic exosomes containing miRNA signals.

Methods

Experiments like CCK-8, IC50, and flow cytometry were used to determine how hypoxic exosomes affect the sorafenib sensitivity of normoxic hepatocellular carcinoma cells. A high-throughput sequencing approach was employed to ide

SUBMITTER: Shu B 

PROVIDER: S-EPMC12659515 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature