WSSV-induced reversal of the malate-aspartate shuttle facilitates viral replication in shrimp hemocytes.

ABSTRACT:

Background

White spot syndrome virus (WSSV), one of the most devastating pathogens in global shrimp aquaculture, has been shown to hijack and reprogram host metabolic pathways to support its replication. Among the various host metabolic circuits, the malate-aspartate shuttle (MAS) is a key redox-balancing mechanism that facilitates the translocation of cytosolic NADH into mitochondria, thereby sustaining glycolysis and mitochondrial function. To date, however, the involvement of MAS in WSSV pathogenesis has not been documented.

Methods

In this study, we investigated the role of the MAS pathway in WSSV replication. We first assessed the mRNA level changes of MAS-related genes in WSSV infected shrimp. dsRNA-mediated gene silencing was also employed to examine its impact on the

SUBMITTER: Guo FJ 

PROVIDER: S-EPMC12659559 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature