CXCL6 Orchestrates Macrophage-Driven Inflammation in Diabetic Kidney Disease and Represents a Druggable Target.

ABSTRACT:

Introduction

Diabetic kidney disease (DKD) is a leading cause of end-stage renal disease and is increasingly recognized to involve early and progressive tubulointerstitial injury. However, the key molecular drivers of this process and their therapeutic potential remain poorly defined.

Methods

We conducted an integrative analysis of three DKD transcriptomic datasets using weighted gene correlation network analysis and differential expression analysis, followed by single-cell RNA sequencing to localize candidate genes to specific renal cell types. Functional validation was performed using in vitro assays in HK-2 and THP-1 cells, in vivo DKD mouse models, and patient kidney tissue. A virtual screening approach was applied to identify candidate inhibitors.

Results

We iden

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12659677 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature