The RNA-binding protein HNRNPA2B1 regulates neurite RNA abundance and motor-dependent cargo transport.

ABSTRACT: RNA molecules are localized to subcellular regions through interactions between localization-regulatory cis-elements and trans-acting RNA binding proteins (RBPs). However, the identities of RNAs whose localization is regulated by a specific RBP, as well as the impacts of that RNA localization on cell function, have generally remained unknown. Here, we demonstrate that the RBP HNRNPA2B1 acts to keep specific RNAs out of neuronal projections. Using subcellular fractionation, high-throughput sequencing, and single-molecule RNA FISH, we find that hundreds of RNAs demonstrate markedly increased abundance in neurites in HNRNPA2B1 knockout cells. These RNAs often encode motor proteins and are enriched for known HNRNPA2B1 binding sites and motifs in their 3' UTRs. The speed and processivity of mic

SUBMITTER: Lo J 

PROVIDER: S-EPMC12659704 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature