Genome-wide DNA methylation dynamics at "heading" stage of panicle and flag leaf in contrasting rice cultivars under field drought conditions.

ABSTRACT:

Introduction

Drought stress induces widespread genome-wide alterations in DNA methylation of rice. These changes work to alter gene expression and are relatively unexplored in reproductive tissues like flag leaf and panicle under field drought conditions. This study aims to explore the same in the panicle and flag leaf tissue of IR64 (drought-sensitive) and N22 (drought-tolerant) rice cultivars under field-drought conditions during the 'heading' stage of development.

Methods

For the same, we generated whole-genome bisulfite sequencing libraries from the corresponding tissues and analysed them in detail.

Results and discussion

The DNA methylation dynamics in adult tissue (flowering stage) was found to be clearly distinct from that of the seedling stage. Further, the co

SUBMITTER: Jajo R 

PROVIDER: S-EPMC12659711 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature